Journal: PLoS ONE

Article Title: Potential Relevance of α 1 -Adrenergic Receptor Autoantibodies in Refractory Hypertension

doi: 10.1371/journal.pone.0003742

Figure Lengend Snippet: (A) α 1 -AAB isolated from the serum of a patient with refractory hypertension elicited a Ca 2+ signal. (B) The rabbit α 1 -AB gave a similar Ca 2+ signal. (C) A human control IgG preparation was unable to affect intracellular Ca 2+ . Cardiomyocytes were electrically stimulated at 1 Hz, and the peak Ca 2+ was monitored.

Article Snippet: Following, vessel rings were incubated with α 1 -AAB (5 µg in 5 ml PBS buffer), rabbit α 1 -AB (50 µg in 5 ml PBS buffer), rabbit IgG (50 µg in 5 ml PBS buffer, Dunn Labortechnik GmBH, Asbach, Germany) and human control IgG endobulin (50 µg in 5 ml PBS buffer).

Techniques: Isolation

Journal: PLoS ONE

Article Title: Potential Relevance of α 1 -Adrenergic Receptor Autoantibodies in Refractory Hypertension

doi: 10.1371/journal.pone.0003742

Figure Lengend Snippet: A brisk KCl response is documented. (A) Representative experiment showing the contractile response to phenylephrine (PE, 10 nM–10 µM) and rabbit α 1 -AB (50 µg in 5 ml PBS buffer). The PE and antibody responses were similar in kind. (B) Contractile response of human α 1 -AAB isolated from two patients (5 µg in 5 ml PBS buffer) and rabbit α 1 -AB (50 µg in 5 ml PBS buffer) are compared to the PE response (300 nM and 10 µM, respectively) is shown. Contractions are expressed as % KCl response from patient 1 and patient 2. The human antibody responses were less than the low and high-dose PE responses. The rabbit α 1 -AB responses approached the PE responses. Two columns on the right are control human and control rabbit IgG showing no response.

Article Snippet: Following, vessel rings were incubated with α 1 -AAB (5 µg in 5 ml PBS buffer), rabbit α 1 -AB (50 µg in 5 ml PBS buffer), rabbit IgG (50 µg in 5 ml PBS buffer, Dunn Labortechnik GmBH, Asbach, Germany) and human control IgG endobulin (50 µg in 5 ml PBS buffer).

Techniques: Isolation